Cheap clean-up for PCR: View protocol as .pdf

This is a cheap alternative for cleaning up PCR reactions prior to direct sequencing. It requires that your PCR reaction is very specific (i.e., no extraneous bands or primer dimer). You’ll also want to have pretty strong product, as you do lose significantly more DNA than with most kits. This can be a helpful alternative if your conditions are right and you’re running low on money (and who isn’t?).

Cheap clean-up for sequencing:View protocol as .pdf

Another cheap alternative to the kits. Make sure the sample has COMPLETELY dried before proceeding to prep it for your sequencer (a good whiff should tell you whether ethanol is still present).

DNA Isolation-PCI:View protocol as .pdf

This is a variation on the standard phenol-chloroform DNA isolation protocol from Sambrook et al. (1989). I used this protocol to isolate total genomic DNA from small (3 mm) pieces of bat wing tissue. Typical yield is 400-2000 ng of DNA.